Puki baasal matam pangambangan [[duktus Muller]] di embrio.<ref>{{cite journal| author=Cai Y| title=Revisiting old vaginal topics: conversion of the Müllerian vagina and origin of the "sinus" vagina.|journal=Int J Dev Biol 2009; 53:925-34| pmid=19598112| doi=10.1387/ijdb.082846yc| volume=53| issue=7| year=2009| pages=925–34}}</ref> Puki manusia marupakan sabuting saluran otot nang maumat nang mamanjang mata [[gulu rahim]] sampai ka [[vulva]].<ref name="Snell">{{cite book|last=Snell|first=Richard S.|title=Clinical Anatomy: An Illustrated Review with Questions and Explanations|url=http://books.google.com/books?id=5s7jDVQkCfoC&pg=PA98|accessdate=19 February 2014|year=2004|publisher=Lippincott Williams & Wilkins|isbn=978-0-7817-4316-7|page=98}}</ref> Warna puki lawan bagian dalam vulva adalah habang jambu kahabangan, wan vagina mahubungakan vulva supervisial lawan gulu rahim (serviks) matan [[uterus]] di subalah dalam. Puki, nang baandak di balakang (posterior) uretra wan kandung kamih, sacara superior mancapai [[perineum]] wan sacara posterior manuju gulu rahim; gulu rahim wan vagina tahubung malalui sudut nang babelok, yaitu sakitar 90 darajat.<ref name="Mulhall">{{cite book|first = John P.|last = Mulhall|editors=John P. Mulhall, Luca Incrocci, Irwin Goldstein, Ray Rosen|title = Cancer and Sexual Health|isbn = 1-60761-915-6|publisher = [[Springer Publishing|Springer]]|year = 2011|pages=13–22|accessdate = February 21, 2014|url = http://books.google.com/?id=GpIadil3YsQC&pg=PA13&dq=#v=onepage&q&f=false}}</ref>
Lapisan internal vagina tadiri matan [[epitel skuamosa berlapis]].<ref name="Wylie">{{cite book|last=Wylie|first=Linda|title=Essential Anatomy and Physiology in Maternity Care|accessdate=19 February 2014|year=2005|publisher=Elsevier Health Sciences|isbn=0-443-10041-1|pages=157–158|url=http://books.google.com/books?id=QgpOvSDxGGYC&pg=PA157}}</ref> Di bawah lapisan ngini adalah lapisan [[otot polos]], nang kawa maulah puki kontraksi salawas basakian wan haratan baranak. Di bawah otot adalah lapisan [[jaringan ikat]] nang disambat [[adventitia]].<ref name="Wheater">{{Cite book|editor-last=Young|editor-first=B|title=Wheater's Functional Histology: A Text and Colour Atlas|url=https://archive.org/details/wheatersfunction00youn|publisher=[[Elsevier]]|year=2006|page=[https://archive.org/details/wheatersfunction00youn/page/n852 377]|edition=5th|isbn=978-0443068508 }}</ref>
